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Anti-PRPH Antibody
Catalog #: 144-04048
PRPH Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P41219 |
| Gene Id | 5630 |
| Gene Symbols | PRPH |
| Protein Name / Synonyms | PRPH, NEF4, PRPH1, peripherin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.
Dilutions
WB 1:500 - 1:2000
IHC 1:20 - 1:200
IHC 1:20 - 1:200
Format
Calculated MW
53kDa
Observed MW
62kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPH (NP_006253.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRPH antibody (144-04048) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using PRPH antibody (144-04048) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PRPH antibody (144-04048) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.