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Anti-PSA / KLK3 Antibody
Catalog #: 130-10069
Rabbit Anti-Human PSA / KLK3 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P07288 |
| Gene Id | 354 |
| Gene Symbols | KLK3 APS |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from human KLK3. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein KLK3. The membrane blot was probed with antiKLK3 primary antibody (1 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. |
| Antigen Catalog Number | 230-00044 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Kallikrein-3 (Prostate-specific antigen, PSA, KLK3) is a glycoprotein of approximately 30 kDa found mainly in prostatic tissue and seminal fluid. KLK3 is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostate disorders. KLK3 function in the liquefaction of seminal coagulum, through hydrolysis of the high molecular mass seminal vesicle protein. Its substrates includes semenogelin I & II, fibronectin, insulin-like growth factor binding protein 3 (IGBP3), and laminin. Highly expressed by normal and malignant prostate cancer cells, PSA has been implicated in the growth, invasion, and metastasis of prostate cancer cells. Therefore, KLK3 is widely used as a diagnostic marker for prostate cancer.
Specificity
This antibody binds to Human KLK3. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilizedantibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Native Human Prostate Specific Antigen (catalog #: DS-01-0370)Mouse Anti-Human Prostate Specific Antigen (catalog #: DS-MB-02172)
References
- Emami N, et al. (2008) Utility of kallikrein-related peptidases (KLKs) as cancer biomarkers. Clin Chem. 54(10):1600-7.
- Lundwall A, et al. (2008) Kallikrein-related peptidases. Cell Mol Life Sci. 65(13):2019-38.
Expiration:
12 months from the date of shipment when stored properly.