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Anti-PSA/KLK3 Antibody, Biotinylated
Catalog #: 130-10069B-50
Rabbit anti-PSA/KLK3 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P07288 |
| Gene Id | 354 |
| Gene Symbols | KLK3 APS |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from human KLK3. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein KLK3. The membrane blot was probed with antiKLK3 primary antibody (1 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Kallikrein-3 (Prostate-specific antigen, PSA, KLK3) is a glycoprotein of approximately 30 kDa found mainly in prostatic tissue and seminal fluid. KLK3 is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostate disorders. KLK3 function in the liquefaction of seminal coagulum, through hydrolysis of the high molecular mass seminal vesicle protein. Its substrates includes semenogelin I & II, fibronectin, insulin-like growth factor binding protein 3 (IGBP3), and laminin. Highly expressed by normal and malignant prostate cancer cells, PSA has been implicated in the growth, invasion, and metastasis of prostate cancer cells. Therefore, KLK3 is widely used as a diagnostic marker for prostate cancer.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to Human KLK3. Cross reactivity with mouse and rat are expected from sequence similarity.
Related Products
Native Human Prostate Specific Antigen (catalog #: DS-01-0370)Mouse Anti-Human Prostate Specific Antigen (catalog #: DS-MB-02172)
References
- Emami N, et al. (2008) Utility of kallikrein-related peptidases (KLKs) as cancer biomarkers. Clin Chem. 54(10):1600-7.
- Lundwall A, et al. (2008) Kallikrein-related peptidases. Cell Mol Life Sci. 65(13):2019-38.
Expiration:
12 months from the date of shipment when stored properly.