Synonyms
FLJ00245 antibody, GLBA antibody, MGC110993 antibody, SAP1 antibody, PSAP antibody, sphingolipid activator protein-1 antibody, proactivator polypeptide antibody, prosaposin antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
58 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 250 of PSAP (Uniprot ID# P07602)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PSAP(107-10244) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10244 diluted at 1:2000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.