Rabbit Anti-PSMB1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P20618 |
| Gene Id | 5689 |
| Gene Symbols | PSMB1 |
| Protein Name / Synonyms | PSMB1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PSMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-241 amino acids from the C-terminal region of human PSMB1. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PSMB1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
Specificity
Other Names
PSMB1; PSC5; Proteasome subunit beta type-1; Macropain subunit C5; Multicatalytic endopeptidase complex subunit C5; Proteasome component C5; Proteasome gamma chain
NCBI Accession #
NP_002784.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
26489
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of PSMB1 Antibody (C-term) (Cat# 102-18057) in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35ug/lane). PSMB1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of PSMB1 Antibody (C-term)(Cat# 102-18057) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). |
 | PSMB1 Antibody (C-term) (Cat# 102-18057) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.