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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5702 |
| Protein Name / Synonyms | PSMC3 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC8487 antibody, TBP1 antibody, PSMC3 antibody, TBP-1 antibody, human immunodeficiency virus tat transactivator binding protein-1 antibody, proteasome subunit P50 antibody, proteasome 26S subunit ATPase 3 antibody, 26S proteasome AAA-ATPase subunit RPT5 antibody, 26S protease regulatory subunit 6A antibody, Tat-binding protein 1 antibody, "proteasome (prosome, macropain) 26S subunit, ATPase, 3 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
49 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 51 and 361 of PSMC3 (Uniprot ID# P17980)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMC3(107-10855) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PSMC3(107-10855) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: MOLT4
10% SDS PAGE
107-10855 diluted at 1:5000
A: MOLT4
10% SDS PAGE
107-10855 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.