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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5706 |
| Protein Name / Synonyms | PSMC6 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CADP44 antibody, MGC12520 antibody, P44 antibody, SUG2 antibody, p42 antibody, PSMC6 antibody, conserved ATPase domain protein 44 antibody, proteasome 26S subunit ATPase 6 antibody, 26S proteasome AAA-ATPase subunit RPT4 antibody, proteasome subunit p42 antibody, 26S protease regulatory subunit 10B antibody, 26S protease regulatory subunit S10B antibody, "proteasome (prosome, macropain) 26S subunit, ATPase, 6 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 301 of PSMC6 (Uniprot ID# P62333)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSMC6(107-10446) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PSMC6(107-10446) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10446 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-10446 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
107-10446 diluted at 1:500
A: 293T
7.5% SDS PAGE
107-10446 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.