Anti-PSME1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q06323
Gene Id	5720
Gene Symbols	PSME1
Protein Name / Synonyms	PSME1 (C-term)
Clonality	Polyclonal
Preparation	This PSME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the C-terminal region of human PSME1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig, Monkey

Other Names

PSME1; IFI5111; Proteasome activator complex subunit 1; 11S regulator complex subunit alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; Proteasome activator 28 subunit alpha

NCBI Accession #

NP_006254.1

Other Accession #

Q63797; Q64L94; P97371; P58238; Q4U5R3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28723

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	PSME1 Antibody (C-term)（Cat# 102-16452）western blot analysis in HL-60 cell line lysates (35ug/lane). This demonstrates the PSME1 antibody detected the PSME1 protein (arrow).
	PSME1 Antibody (C-term) (Cat# 102-16452) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSME1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	PSME1 Antibody (C-term) (Cat# 102-16452) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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