Anti-PSME2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UL46
Gene Id	5721
Gene Symbols	PSME2
Protein Name / Synonyms	PSME2 (C-term)
Clonality	Polyclonal
Preparation	This PSME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the C-terminal region of human PSME2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 is the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

PSME2; Proteasome activator complex subunit 2; 11S regulator complex subunit beta; Activator of multicatalytic protease subunit 2; Proteasome activator 28 subunit beta

NCBI Accession #

NP_002809.2

Other Accession #

Q63798; P97372; Q5E9G3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27402

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from HepG2 cell line, using PSME2 Antibody (C-term)(Cat# 102-16890). 102-16890 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	PSME2 Antibody (C-term) (Cat# 102-16890) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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