Synonyms
PI31 antibody, PSMF1 antibody, hPI31 antibody, proteasome inhibitor PI31 subunit antibody, proteasome inhibitor hP131 subunit antibody, "proteasome (prosome, macropain) inhibitor subunit 1 (PI31) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
30 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 209 and 271 of PSMF1 (Uniprot ID# Q92530)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PSMF1 antibody [C2C3], C-term detects PSMF1 protein at Cytoplasm and Nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSMF1 protein stained by PSMF1 antibody [C2C3], C-term (107-10480) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10480 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.