Anti-PTGS1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P23219
Gene Id	5742
Gene Symbols	PTGS1
Protein Name / Synonyms	PTGS1 (C-term)
Clonality	Polyclonal
Preparation	This PTGS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human PTGS1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis.

Specificity

Other Names

PTGS1; COX1; Prostaglandin G/H synthase 1; Cyclooxygenase-1; Prostaglandin H2 synthase 1; Prostaglandin-endoperoxide synthase 1

NCBI Accession #

NP_000953.2;NP_001258094.1;NP_001258095.1;NP_001258297.1;NP_542158.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68686

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	PTGS1 Antibody (C-term) (Cat# 102-15165) western blot analysis in A431,K562 cell line lysates (35ug/lane).This demonstrates the PTGS1 antibody detected the PTGS1 protein (arrow).
	Fluorescent image of Hela cell stained with PTGS1 Antibody (C-term)(Cat# 102-15165).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTGS1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).PTGS1 immunoreactivity is localized to Golgi significantly.
	Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PTGS1 antibody (C-term) (Cat# 102-15165), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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