Anti-PTK2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q05397
Gene Id	5747
Gene Symbols	PTK2
Protein Name / Synonyms	PTK2 (Center)
Clonality	Polyclonal
Preparation	This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

Specificity

Predicted Reactivity

Rat, Chicken

Other Names

PTK2; FAK; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

NCBI Accession #

NP_001186578.1;NP_722560.1

Other Accession #

O35346; Q00944

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

119233

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of PTK2 Antibody (Center) (Cat# 102-17947) in T47D cell line lysates (35ug/lane). PTK2 (arrow) was detected using the purified Pab.
	Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (Center) (Cat# 102-17947). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of K562 cells using PTK2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of PTK2 Antibody (Center)(Cat# 102-17947) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).