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Anti-PTPN2 Antibody
Catalog #: 144-01808
PTPN2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17706 |
| Gene Id | 5771 |
| Gene Symbols | PTPN2 |
| Protein Name / Synonyms | PTPN2, PTN2, PTPT, TC-PTP, TCELLPTP, TCPTP, tyrosine-protein phosphatase non-receptor type 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
40kDa/45kDa/48kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human PTPN2 (NP_536348.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PTPN2 antibody (144-01808) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat heart using PTPN2 antibody (144-01808) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PTPN2 antibody (144-01808). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.