Anti-PTPN6 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P29350
Gene Id	5777
Gene Symbols	PTPN6
Protein Name / Synonyms	PTPN6 (Center)
Clonality	Polyclonal
Preparation	This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

Specificity

Other Names

PTPN6; HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1

NCBI Accession #

NP_002822.2;NP_536858.1;NP_536859.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

67561

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:10-50

	Immunofluorescent analysis of U251 cells, using PTPN6 Antibody (Center) (Cat# 102-15158). 102-15158 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
	Western blot analysis of PTPN6 Antibody (Center) (Cat# 102-15158) in HL-60 cell line lysates (35ug/lane).PTPN6 (arrow) was detected using the purified Pab.
	Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center) (Cat# 102-15158). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2) (Origene Technologies).(2ug/ml)
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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