PUF60 antibody, 0.81 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 22827 |
| Protein Name / Synonyms | PUF60 |
| Concentration (lot specific) | 0.81 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FIR antibody, FLJ31379 antibody, RoBPI antibody, SIAHBP1 antibody, PUF60 antibody, poly(U)-binding-splicing factor PUF60 antibody, Ro ribonucleoprotein-binding protein 1 antibody, ro-binding protein 1 antibody, siah-BP1 antibody, FBP interacting repressor antibody, roBP1 antibody, poly-U binding splicing factor PUF60 antibody, Ro ribonucleoprotein binding protein 1 antibody, siah binding protein 1 antibody, 60 kDa poly(U)-binding-splicing factor antibody, siah-binding protein 1 antibody, fuse-binding protein-interacting repressor antibody, pyrimidine tract binding splicing factor antibody, FBP-interacting repressor antibody, poly-U binding splicing factor 60KDa antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
60 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 346 and 523 (Q9UHX1) of PUF60
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PUF60(107-10311) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded oral tissue, using PUF60(107-10311) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:Hep G2
B:MOLT4
C: Raji
7.5% SDS PAGE
107-10311 diluted at 1:500
A:Hep G2
B:MOLT4
C: Raji
7.5% SDS PAGE
107-10311 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.