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Anti-Pyruvate Dehydrogenase E1 alpha Antibody
Catalog #: 107-10422
Pyruvate Dehydrogenase E1 alpha antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5160 |
| Protein Name / Synonyms | Pyruvate Dehydrogenase E1 alpha |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Energy Metabolism, Glycolysis & Lipid Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
PDHA antibody, PDHCE1A antibody, PHE1A antibody, PDHA1 antibody, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody, pyruvate dehydrogenase complex, E1-alpha polypeptide 1 antibody, PDHE1-A type I antibody, pyruvate dehydrogenase (lipoamide) alpha 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 39 and 325 of (Uniprot ID# P08559)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Pyruvate Dehydrogenase E1 alpha antibody (107-10422) dilution: 1:500.
Sample: Paraffin-embedded hepatoma.
Pyruvate Dehydrogenase E1 alpha antibody (107-10422) dilution: 1:500.
Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
107-10422 diluted at 1:5000
A: mouse brain
10% SDS PAGE
107-10422 diluted at 1:5000
Sample (50 ug of whole cell lysate)
A: Rat brain
10% SDS PAGE
107-10422 diluted at 1:3000
A: Rat brain
10% SDS PAGE
107-10422 diluted at 1:3000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha(107-10422) antibody at 1:200 dilution.
Sample(30 µg of whole cell lysate)
A:Hep G2
B:MOLT4
12% SDS PAGE
107-10422 diluted at 1:1000
A:Hep G2
B:MOLT4
12% SDS PAGE
107-10422 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.