Anti-Pyruvate Kinase (liver/RBC) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	5313
Protein Name / Synonyms	Pyruvate Kinase (liver/RBC)
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Phosphorylation, Energy Metabolism, Glycolysis & Lipid Metabolism
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

PK1 antibody, PKL antibody, PKR antibody, PKRL antibody, RPK antibody, PKLR antibody, R-type/L-type pyruvate kinase antibody, pyruvate kinase type L antibody, red cell/liver pyruvate kinase antibody, pyruvate kinase 1 antibody, pyruvate kinase isozyme R/L antibody, "pyruvate kinase, liver and blood cell antibody", pyruvate kinase isozymes R/L antibody, "pyruvate kinase, liver and RBC antibody"

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

62 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 260 of Pyruvate Kinase (liver/RBC) (Uniprot ID# P30613)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody (107-10974) diluted at 1:500.
Blue: Hoechst 33343 staining.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC)(107-10974) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
E: A375
7.5% SDS PAGE
107-10974 diluted at 1:1000

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (107-10974) dilution: 1:10000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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