Anti-Pyruvate Kinase (PKM2) (C-term) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Non-Human Primate (rhesus monkey)
Host Species	Rabbit
Accession Number	P14618
Gene Id	5315
Gene Symbols	PKM
Protein Name / Synonyms	Pyruvate Kinase (PKM2) (C-term)
Clonality	Polyclonal
Preparation	This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Phosphorylation, Energy Metabolism, Glycolysis & Lipid Metabolism
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Specificity

Other Names

PKM; OIP3; PK2; PK3; PKM2; Pyruvate kinase isozymes M1/M2; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Tumor M2-PK; p58

NCBI Accession #

NP_001193725.1;NP_001193726.1;NP_001193727.1;NP_001193728.1;NP_002645.3;NP_872270.1;NP_872271.1

Other Accession #

P14786

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

57937

Recommended Dilutions

IF: 1:200; WB: 1:1000; IHC: 1:50-1:100

	Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-17097 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
	Western blot analysis of anti-h PKM2-N491 Pab (Cat# 102-17097) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab.
	Pyruvate Kinase (PKM2) Antibody (C-term) (Cat# 102-17097) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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