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Anti-PYY Antibody, Biotinylated
Catalog #: RB-08-0011B-50
Rabbit anti-PYY Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NM_004160 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human PYY. This antibody was produced from a rabbit immunized with synthetic peptide. The IgG fraction was purified from rabbit serum by ammonium sulfate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1 µg/ml (detects 2-5 µg/ml original immunogen)), Western Blotting (recommended work dilution= 1:1000-5000 (at least)), Enzyme Immunoassay (EIA) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Peptide YY is a 36 amino acid peptide released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36.
There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which is the most common form of circulating PYY. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide. Circulating PYY concentration increases postprandially and decreases by fasting. Studies on PYY-knockout mice have shown that they have higher fat mass and lower glucose tolerance when compared to control mice, indicating that PYY also plays very important role in energy homeostasis by balancing the food intake.
There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which is the most common form of circulating PYY. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide. Circulating PYY concentration increases postprandially and decreases by fasting. Studies on PYY-knockout mice have shown that they have higher fat mass and lower glucose tolerance when compared to control mice, indicating that PYY also plays very important role in energy homeostasis by balancing the food intake.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human PYY antibody specifically detects target derived both from human recombinant and cellular proteins at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
References
- Murphy KG, Bloom SR (2006). "Gut hormones and the regulation of energy homeostasis". Nature 444 (7121): 8549. PMID 17167473. Batterham RL, Cohen MA, Ellis SM, Le Roux CW, Withers
- DJ, Frost GS, Ghatei MA, Bloom SR (2003). "Inhibition of food intake in obese subjects by peptide YY3-36". The New England journal of medicine 349 (10): 9418. PMID 12954742
Expiration:
12 months from the date of shipment when stored properly.