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Anti-PYY Antibody
Catalog #: RB-08-0011
Rabbit Anti-Human PYY Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_004160 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human PYY. This antibody was produced from a rabbit immunized with synthetic peptide. The IgG fraction was purified from rabbit serum by ammonium sulfate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1 ng/ml (detects 2-5 ng/ml original immunogen)), Western Blotting (recommended work dilution= 1:1000-5000 (at least)), Enzyme Immunoassay (EIA) |
| Reconstitution | Supplied as 200 µg of purified and lyophilized antibody. The antibody is stable for at least one year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggested with sterile water) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 200 µg/ml. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Peptide YY is a 36 amino acid peptide released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36.
There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which is the most common form of circulating PYY. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide. Circulating PYY concentration increases postprandially and decreases by fasting. Studies on PYY-knockout mice have shown that they have higher fat mass and lower glucose tolerance when compared to control mice, indicating that PYY also plays very important role in energy homeostasis by balancing the food intake.
There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which is the most common form of circulating PYY. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide. Circulating PYY concentration increases postprandially and decreases by fasting. Studies on PYY-knockout mice have shown that they have higher fat mass and lower glucose tolerance when compared to control mice, indicating that PYY also plays very important role in energy homeostasis by balancing the food intake.
Specificity
The rabbit anti-human PYY antibody specifically detects target derived both from human recombinant and cellular proteins at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C to -70°C if not intended for use within a month. Otherwise keep at 4°C and minimize freezing and thawing.
References
- Murphy KG, Bloom SR (2006). "Gut hormones and the regulation of energy homeostasis". Nature 444 (7121): 8549. PMID 17167473. Batterham RL, Cohen MA, Ellis SM, Le Roux CW, Withers
- DJ, Frost GS, Ghatei MA, Bloom SR (2003). "Inhibition of food intake in obese subjects by peptide YY3-36". The New England journal of medicine 349 (10): 9418. PMID 12954742
Expiration:
12 months from the date of shipment when stored properly.