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Anti-Rab5 (C-term) Antibody

Catalog #: 102-17043

Rabbit Anti-Human Rab5 (C-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P20339
Gene Id
5868
Gene Symbols
RAB5A
Protein Name / Synonyms
Rab5 (C-term)
ClonalityPolyclonal
PreparationThis Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human Rab5.
Concentration (lot specific)
0.440 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, IF, FC
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Rab5 is required for the fusion of plasma membranes and early endosomes.

Specificity

Other Names
RAB5A; RAB5; Ras-related protein Rab-5A
NCBI Accession #
NP_004153.2

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
23659
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
Western blot analysis of Rab5 Antibody (C-term) (Cat# 102-17043) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Rab5 Antibody (C-term) (Cat# 102-17043) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.