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Anti-RACGAP1 (N-term) Antibody
Catalog #: 107-11052
RACGAP1 antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 29127 |
| Protein Name / Synonyms | RACGAP1, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
HsCYK-4 antibody, ID-GAP antibody, MgcRacGAP antibody, RACGAP1 antibody, male germ cell RacGap antibody, rac GTPase-activating protein 1 antibody, protein CYK4 homolg antibody, GTPase activating protein antibody, Rac GTPase activating protein 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
71 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 202 of RACGAP1 (Uniprot ID# Q9H0H5)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1(107-11052) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1(107-11052) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
107-11052 diluted at 1:1000
A: mouse heart
7.5% SDS PAGE
107-11052 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1(107-11052) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
107-11052 diluted at 1:1000
A: A431
B: Raji
7.5% SDS PAGE
107-11052 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.