Anti-RAD17 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75943
Gene Id	5884
Gene Symbols	RAD17
Protein Name / Synonyms	RAD17 (Center)
Clonality	Polyclonal
Preparation	This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Specificity

Other Names

RAD17; R24L; Cell cycle checkpoint protein RAD17; RF-C/activator 1 homolog

NCBI Accession #

NP_001265551.1;NP_002864.1;NP_579916.1;NP_579917.1;NP_579918.1;NP_579919.1;NP_579920.1;NP_579921.1;NP_579922.1

Other Accession #

NP_579916.1; NP_002864.1; NP_579917.1; NP_579918.1; NP_579922.1; NP_579920.1; NP_579919.1; N

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

77055

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	RAD17 Antibody (Center) (Cat# 102-11214) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).
	RAD17 Antibody (Center) (Cat# 102-11214) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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