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Anti-RAD23B Antibody
Catalog #: 144-01034
RAD23B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P54727 |
| Gene Id | 5887 |
| Gene Symbols | RAD23B |
| Protein Name / Synonyms | RAD23B, HHR23B, HR23B, P58, UV excision repair protein RAD23 homolog B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
35kDa/43kDa
Observed MW
60kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAD23B (NP_002865.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RAD23B antibody (144-01034) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using RAD23B antibody (144-01034) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using RAD23B antibody (144-01034) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RAD23B antibody (144-01034) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RAD23B antibody (144-01034) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.