Anti-Rad54 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	546
Protein Name / Synonyms	Rad54, C-term
Concentration (lot specific)	0.6 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ATR2 antibody, MGC2094 antibody, MRXHF1 antibody, RAD54 antibody, RAD54L antibody, SFM1 antibody, SHS antibody, XH2 antibody, XNP antibody, ZNF-HX antibody, ATRX antibody, ATP-dependent helicase ATRX antibody, "alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae) antibody", Zinc finger helicase antibody, X-linked helicase II antibody, "helicase 2, X-linked antibody", DNA dependent ATPase and helicase antibody, X-linked nuclear protein antibody, transcriptional regulator ATRX antibody, RAD54 homolog antibody, alpha thalassemia/mental retardation syndrome X-linked antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

283 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 2161 and 2443 of Rad54 (Uniprot ID# P46100)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ATRX antibody detects ATRX protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody (107-10253) diluted by 1:1000.

Rad54 antibody [C3], C-term detects Rad54 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell extract
5 % SDS-PAGE
Rad54 antibody [C3], C-term (107-10253) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATRX(107-10253) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ATRX(107-10253) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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