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Anti-RAD9A Antibody
Catalog #: 102-26536
Mouse Anti-RAD9A Antibody, 100 µl
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Mouse |
| Accession Number | Q99638 |
| Gene Id | 5883 |
| Gene Symbols | RAD9A |
| Protein Name / Synonyms | Cell cycle checkpoint control protein;Cell cycle checkpoint control protein RAD9A;DNA repair exonuclease rad9 homolog A;hRAD 9;hRAD9;Rad 9;RAD 9A;RAD9 (S pombe) homolog;RAD9 homolog A;RAD9 homolog;RAD9A;RAD9A_HUMAN. |
| Clonality | Monoclonal |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Other Information
Antigen Type
Recombinant Protein
Images
WB (1:500)
Western blot detection of RAD9A in Hela,MCF7,3T3,COS7 and Rat testis cell lysates using RAD9A mouse mAb (1:500 diluted).Predicted band size:43KDa.Observed band size:55KDa.
IP (1:500)
Immunoprecipitation analysis of Hela and 3T3 cell lysates using RAD9A mouse mAb.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.