Anti-RAF1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04049
Gene Id	5894
Gene Symbols	RAF1
Protein Name / Synonyms	RAF1 (N-term)
Clonality	Polyclonal
Preparation	This RAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RAF1.
Concentration (lot specific)	0.440 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Specificity

Other Names

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; Raf-1

NCBI Accession #

NP_002871.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

73052

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of anti-RAF1 Pab (Cat# 102-17641) in Jurkat cell lysate. RAF1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Confocal immunofluorescent analysis of RAF1 Antibody (N-term)(Cat# 102-17641) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	RAF1 Antibody (N-term) (Cat# 102-17641) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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