Anti-RAG2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	5897
Protein Name / Synonyms	RAG2
Concentration (lot specific)	0.37 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

RAG-2 antibody, RAG2 antibody, V(D)J recombination-activating protein 2 antibody, recombination activating gene 2 antibody

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

59 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID# P55895)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RAG2 antibody detects RAG2 protein by Western blot analysis.
A. 50 µg Rat thymus lysate/extract
7.5 % SDS-PAGE
RAG2 antibody (107-10861) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(107-10861) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(107-10861) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10861 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products