RANKL antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8600 |
| Protein Name / Synonyms | RANKL, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, osteoprotegerin ligand antibody, receptor activator of nuclear factor kappa-B ligand antibody, receptor activator of nuclear factor kappa B ligand antibody, tumor necrosis factor ligand superfamily member 11 antibody, osteoclast differentiation factor antibody, TNF-related activation-induced cytokine antibody, "tumor necrosis factor (ligand) superfamily, member 11 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
35 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 253 and 317 of RANKL (Uniprot ID# O14788)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RANKL(107-10773) antibody at 1:100 dilution.
RANKL antibody [C2C3], C-term detects RANKL protein by western blot analysis.
A. 50 µg rat lung extract
10 % SDS-PAGE
RANKL antibody [C2C3], C-term (107-10773) dilution: 1:1000
A. 50 µg rat lung extract
10 % SDS-PAGE
RANKL antibody [C2C3], C-term (107-10773) dilution: 1:1000
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
RANKL (CD254) antibody
107-10773 diluted at 1:1000
A: Hela
10% SDS PAGE
RANKL (CD254) antibody
107-10773 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.