Anti-RARB (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P10826
Gene Id	5915
Gene Symbols	RARB
Protein Name / Synonyms	RARB (Center)
Clonality	Polyclonal
Preparation	This RARB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the Central region of human RARB.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Chicken

Other Names

RARB; HAP; NR1B2; Retinoic acid receptor beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon

NCBI Accession #

NP_000956.2;NP_057236.1

Other Accession #

P22605; P22448; NP_000956.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50489

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

RARB Antibody (Center) (Cat# 102-13705) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RARB antibody detected the RARB protein (arrow).

Fluorescent confocal image of Hela cell stained with RARB Antibody (Center)(Cat# 102-13705). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RARB primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RARB immunoreactivity is localized to nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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