Anti-RASGRP4 (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	115727
Protein Name / Synonyms	RASGRP4, N-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

RASGRP4 antibody, RAS guanyl-releasing protein 4 antibody, guanyl nucleotide releasing protein 4 antibody, RAS guanyl releasing protein 4 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

75 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 108 and 200 of RASGRP4 (Uniprot ID# Q8TDF6)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4(107-10475) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
107-10475 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using RASGRP4 (107-10475) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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