Anti-Rat TNF-alpha Antibody

Catalog #: DS-PB-01977

Rabbit Anti-Rat TNF-alpha

Product Description

Specifications

Size	0.1 mg
Estimated Lead Time	1-2 weeks
Species	Rat
Specificity	This product recognizes rat tumour necrosis factor alpha (TNF alpha). Rat TNF-alpha is a 17kD cytokine which is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.
Host Species	Rabbit
Protein Name / Synonyms	TNF-alpha / TNF
Preparation	Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Concentration (lot specific)	IgG concentration 1.0 mg/ml after reconstitution.
Recommended Applications	ELISA (dilution: 0.1, 0.2 ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Reconstitution	Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Ambient temperature
Storage	-20°C

Description

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide

Format Type:

Purified

Preparation

Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:

Recombinant rat TNF alpha.

Preservative:

None present

Expiration:

12 months from the date of shipment when stored properly.

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