Anti-RB1 (S608) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P06400
Gene Id	5925
Gene Symbols	RB1
Protein Name / Synonyms	RB1 (S608)
Clonality	Polyclonal
Preparation	This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from human RB1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer.[1] One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins.[2][3] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.

Specificity

Other Names

RB1; Retinoblastoma-associated protein; p105-Rb; pRb; pp110

NCBI Accession #

NP_000312.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

106159

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	Rb Antibody (S608) (Cat# 102-16706) western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstrates the Rb antibody detected the Rb protein (arrow).
	Confocal immunofluorescent analysis of RB1 Antibody (S608)(Cat# 102-16706) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	RB1 Antibody (S608) (Cat# 102-16706)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RB1 Antibody (S608) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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