Anti-RCAN1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P53805
Gene Id	1827
Gene Symbols	RCAN1
Protein Name / Synonyms	RCAN1 (N-term)
Clonality	Polyclonal
Preparation	This RCAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human RCAN1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene.

Specificity

Predicted Reactivity

Mouse

Other Names

RCAN1; ADAPT78; CSP1; DSC1; DSCR1; Calcipressin-1; Adapt78; Down syndrome critical region protein 1; Myocyte-enriched calcineurin-interacting protein 1; Regulator of calcineurin 1

NCBI Accession #

NP_004405.3;NP_981962.1;NP_981963.1

Other Accession #

Q9JHG6; NP_004405.3; NP_981963.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28079

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	RCAN1 Antibody (N-term) (Cat# 102-13137) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).
	Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) (Cat# 102-13137). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.
	Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term)(Cat# 102-13137).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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