3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Renilla reniformis Luciferase Antibody
Catalog #: 130-00005
Rabbit Anti-Renilla reniformis Luciferase Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Renilla Reniformis (sea Pansy) |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a recombinant protein of Renilla reniformis luciferase. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Luciferase belongs to the oxidative enzymes used in bioluminescence and is different from the photoprotein. In the luciferase reaction, luciferase acts on the appropriate substrate luciferin and light is emitted. A variety of organisms regulate their light production using different luciferases. The well studied luciferases are mainly from fireflies and Renilla.
Specificity
This antibody binds to Renilla reniformis. Cross reactivity with luciferase derived from other species has not yet been tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilizedantibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Recombinant Luciferase Western Blot Control (catalog #: RB-15-0003P-WBC)Purified Recombinant Luciferase (catalog # RB-15-0003P)
References
- Nakatsu T, et al. (2006). "Structural basis for the spectral difference in luciferase bioluminescence". Nature 440 (7082):3726.
- Baldwin TO (1996). "Firefly luciferase: the structure is known, but the mystery remains". Structure 4 (3): 2238.
Expiration:
12 months from the date of shipment when stored properly.