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Anti-Renilla reniformis Luciferase Antibody, Biotinylated
Catalog #: 130-00005B-50
Rabbit anti-Renilla reniformis Luciferase Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Renilla Reniformis (sea Pansy) |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a recombinant protein of Renilla reniformis luciferase. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Luciferase belongs to the oxidative enzymes used in bioluminescence and is different from the photoprotein. In the luciferase reaction, luciferase acts on the appropriate substrate luciferin and light is emitted. A variety of organisms regulate their light production using different luciferases. The well studied luciferases are mainly from fireflies and Renilla.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to Renilla reniformis. Cross reactivity with luciferase derived from other species has not yet been tested.
Related Products
Recombinant Luciferase Western Blot Control (catalog #: RB-15-0003P-WBC)Purified Recombinant Luciferase (catalog # RB-15-0003P)
References
- Nakatsu T, et al. (2006). "Structural basis for the spectral difference in luciferase bioluminescence". Nature 440 (7082):3726.
- Baldwin TO (1996). "Firefly luciferase: the structure is known, but the mystery remains". Structure 4 (3): 2238.
Expiration:
12 months from the date of shipment when stored properly.