Anti-RFC2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P35250
Gene Id	5982
Gene Symbols	RFC2
Protein Name / Synonyms	RFC2 (N-term)
Clonality	Polyclonal
Preparation	This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RFC2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.

Specificity

Predicted Reactivity

Bovine

Other Names

RFC2; Replication factor C subunit 2; Activator 1 40 kDa subunit; Activator 1 subunit 2; Replication factor C 40 kDa subunit

NCBI Accession #

NP_001265720.1;NP_001265721.1;NP_001265722.1;NP_002905.2;NP_852136.1

Other Accession #

Q641W4; Q05B83

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39157

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000; FC: 1:10-50

	Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term)(Cat# 102-15144), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)
	Western blot analysis of anti-RFC2 Antibody (N-term)(Cat# 102-15144) in K562 cell line lysates (35ug/lane). RFC2(arrow) was detected using the purified Pab.
	RFC2 Antibody (N-term) (Cat# 102-15144) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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