RFC4 antibody [N1C3], 0.62 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5984 |
| Protein Name / Synonyms | RFC4 |
| Concentration (lot specific) | 0.62 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
A1 antibody, MGC27291 antibody, RFC37 antibody, RFC4 antibody, A1 37 kDa subunit antibody, activator 1 37 kDa subunit antibody, RF-C 37 kDa subunit antibody, activator 1 subunit 4 antibody, replication factor C 37 kDa subunit antibody, RFC 37 kDa subunit antibody, replication factor C subunit 4 antibody, "replication factor C (activator 1) 4, 37kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 18 and 323 of RFC4 (Uniprot ID# P35249)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10424 diluted at 1:500
A: mouse liver
10% SDS PAGE
107-10424 diluted at 1:500
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(107-10424) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(107-10424) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
10% SDS PAGE
107-10424 diluted at 1:1000
A: H1299
B: Hela
C: Hep G2
10% SDS PAGE
107-10424 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.