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Anti-RFWD2 (C-term) Antibody
Catalog #: 102-14486
Rabbit Anti-Human RFWD2 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8NHY2 |
| Gene Id | 64326 |
| Gene Symbols | RFWD2 |
| Protein Name / Synonyms | RFWD2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Specificity
Predicted Reactivity
Mouse
Other Names
RFWD2; COP1; RNF200; E3 ubiquitin-protein ligase RFWD2; Constitutive photomorphogenesis protein 1 homolog; RING finger and WD repeat domain protein 2; RING finger protein 200
NCBI Accession #
NP_001001740.1;NP_071902.2
Other Accession #
Q9R1A8; NP_071902.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
80474
Recommended Dilutions
WB: 1:1000
 | RFWD2 Antibody (C-term) (Cat# 102-14486) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the RFWD2 antibody detected the RFWD2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.