Rabbit Anti-Human RFX1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P22670 |
| Gene Id | 5989 |
| Gene Symbols | RFX1 |
| Protein Name / Synonyms | RFX1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.
Specificity
Predicted Reactivity
Mouse
Other Names
RFX1; MHC class II regulatory factor RFX1; Enhancer factor C; Regulatory factor X 1; Transcription factor RFX1
NCBI Accession #
NP_002909.4
Other Accession #
P48377; NP_002909.4
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
104758
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat# 102-13130).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).RFX1 immunoreactivity is localized to Nucleus significantly. |
 | RFX1 Antibody (C-term) (Cat# 102-13130) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.