Anti-Ribosome binding protein 1 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	6238
Protein Name / Synonyms	Ribosome binding protein 1, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp586A1420 antibody, ES/130 antibody, ES130 antibody, FLJ36146 antibody, MGC157720 antibody, MGC157721 antibody, hES antibody, RRBP1 antibody, 180 kDa ribosome receptor homolog antibody, ribosome-binding protein 1 antibody, ribosome receptor protein antibody, ES/130-related protein antibody, ribosome binding protein 1 homolog 180kDa (dog) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

152 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1183 and 1410 of Ribosome binding protein 1 (Uniprot ID# Q9P2E9)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(107-10294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(107-10294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.

Ribosome binding protein 1 antibody validation by siRNA knock-down.
Upperpanel: Ribosome binding protein 1 antibody 107-10294
Lower panel: Vinculin antibody
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 µg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5 % SDS-PAGE
Ribosome binding protein 1 antibody (107-10294) dilution: 1:500
Vinculin antibody dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(107-10294) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)

A:NIH-3T3
5% SDS PAGE

107-10294 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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