Anti-RIPK2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O43353
Gene Id	8767
Gene Symbols	RIPK2
Protein Name / Synonyms	RIPK2 (C-term)
Clonality	Polyclonal
Preparation	This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human RIPK2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Specificity

Other Names

RIPK2; CARDIAK; RICK; RIP2; Receptor-interacting serine/threonine-protein kinase 2; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; RIP-like-interacting CLARP kinase; Receptor-interacting protein 2; Tyrosine-protein kinase RIPK2

NCBI Accession #

NP_003812.1

Other Accession #

NP_003812

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

61195

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-RIPK2 Pab (Cat# 102-17642) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Western blot analysis of anti-RIPK2 Pab (Cat# 102-17642) in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.
	Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) (Cat# 102-17642). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIPK2 Antibody (C-term) (Cat# 102-17642), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.