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Anti-RNA polymerase IIB (N-term) Antibody
Catalog #: 107-10342
RNA polymerase IIB antibody [N1], N-Terminus, 0.65 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5431 |
| Protein Name / Synonyms | RNA polymerase IIB, N-term |
| Concentration (lot specific) | 0.65 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, POLR2B antibody, DNA-directed RNA polymerase II 140 kDa polypeptide antibody, DNA-directed RNA polymerase II subunit B antibody, "DNA directed RNA polymerase II 140 kDa polypeptide, RNA polymerase II subunit 2, RNA polymerase II second largest subunit antibody", RNA polymerase II subunit B2 antibody, RNA polymerase II subunit 2 antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, "polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
134 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 172 of RNA polymerase IIB (Uniprot ID# P30876)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B(107-10342) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
107-10342 diluted at 1:1000
A: BCL-1
5% SDS PAGE
107-10342 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B(107-10342) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
107-10342 diluted at 1:1000
A: H1299
5% SDS PAGE
107-10342 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.