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Anti-Roundabout 4 (N-term) Antibody
Catalog #: 102-16409
Rabbit Anti-Human Roundabout 4 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8WZ75 |
| Gene Id | 54538 |
| Gene Symbols | ROBO4 |
| Protein Name / Synonyms | Roundabout 4 (N-term) |
| Clonality | Polyclonal |
| Preparation | This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.
Specificity
Other Names
ROBO4; Roundabout homolog 4; Magic roundabout
NCBI Accession #
NP_061928.4
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
107457
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Roundabout 4 Antibody (N-term) (Cat# 102-16409) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow). |
 | Roundabout 4 Antibody (N-term) (Cat# 102-16409) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Roundabout 4 Antibody (N-term) (Cat# 102-16409) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.