Anti-RPA14 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	6119
Protein Name / Synonyms	RPA14
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

REPA3 antibody, RPA3 antibody, RF-A protein 3 antibody, RP-A p14 antibody, replication protein A 14 kDa subunit antibody, replication factor A protein 3 antibody, replication protein A3, 14kDa antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

14 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 121 of RPA14 (Uniprot ID# P35244)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RPA14 antibody detects RPA14 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody (107-10180) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded lung CA patient tumor, using RPA 14 kDa subunit (107-10180) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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