RPA32 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 6118 |
| Protein Name / Synonyms | RPA32 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
REPA2 antibody, RPA32 antibody, RPA2 antibody, RP-A p34 antibody, replication protein A 32 kDa subunit antibody, RP-A p32 antibody, RF-A protein 2 antibody, replication protein A 34 kDa subunit antibody, replication factor A protein 2 antibody, replication protein A2, 32kDa antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 202 of RPA32 (Uniprot ID# P15927)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
A. 30 µg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (107-11044) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 32 kDa subunit (107-11044) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (107-11044) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
107-11044 diluted at 1:1000
A: 293T
B: A431
12% SDS PAGE
107-11044 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.