Anti-RPA70 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	6117
Protein Name / Synonyms	RPA70
Concentration (lot specific)	0.49 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, RPA1 antibody, single-stranded DNA-binding protein antibody, replication protein A 70 kDa DNA-binding subunit antibody, replication factor A protein 1 antibody, RP-A p70 antibody, RF-A protein 1 antibody, MSTP075 antibody, "replication protein A1, 70kDa antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

68 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (Uniprot ID# P27694)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10795 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (107-10795) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (107-10795) antibody at 1:100 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10795 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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