Anti-RPC8 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y535
Gene Id	171568
Gene Symbols	POLR3H
Protein Name / Synonyms	RPC8 (N-term)
Clonality	Polyclonal
Preparation	This RPC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human RPC8.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RPC8 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. It is acting as nuclear and cytosolic DNA sensor involved in innate immune response. It can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Specificity

Other Names

POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit

NCBI Accession #

NP_001018060.1;NP_001018062.1;NP_612211.1

Other Accession #

Q9D2C6

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22918

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	Western blot analysis of RPC8 Antibody (N-term) (Cat# 102-15802) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.
	RPC8 Antibody (N-term) (Cat# 102-15802) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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