Anti-RPL27A (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Hamster
Host Species	Rabbit
Accession Number	P46776
Gene Id	6157
Gene Symbols	RPL27A
Protein Name / Synonyms	RPL27A (C-term)
Clonality	Polyclonal
Preparation	This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Monkey

Other Names

RPL27A; 60S ribosomal protein L27a

NCBI Accession #

NP_000981.1

Other Accession #

P18445; P14115; Q4R723; Q56K03; NP_000981.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

16561

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	RPL27A Antibody (C-term) (Cat# 102-10861) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
	RPL27A Antibody (C-term) (Cat# 102-10861) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
	RPL27A Antibody (C-term) (Cat# 102-10861) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	RPL27A Antibody (C-term) (Cat# 102-10861) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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