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Anti-RPN1 Antibody
Catalog #: 144-12497
RPN1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04843 |
| Gene Id | 6184 |
| Gene Symbols | RPN1 |
| Protein Name / Synonyms | RPN1, OST1, RBPH1, ribophorin I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
68kDa
Observed MW
69kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RPN1 antibody (144-600827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (144-600827) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (144-600827) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.